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Objective:To study the effects of soybean phospholipids on learning and memory abilities and contents of fatty acids in hippocampus of mice and its mechanism. Method:Mice were randomly divided into four groups:low dose(2.5g/kg) ,middle dose(5.0g/kg) and high dose(10.0g/kg) groups of soybean phospholipids and control group. The treatment groups were fed with soybean phospholipids every day while the control group with distilled water for 30 d.After learning and memory abilities were measured,the mice were killed and contents of fatty acids in hippocampus of mice were determined by high performance liquid chromatography(HPLC) . Results:(1) The learning and memory abilities of mice in middle and high dose groups were significantly improved,but only memory abilities in low dose groups were stronger than control group.(2) The contents of protein in hippocampus in middle and high dose groups were increased extremely.(3) The contents of oleic acid(C18:1) and docosahexaenoic acid(DHA,C22:6 ) were obviously enhanced in hippocampus of high dose group;but decreased in eicosapentaenoic acid(EPA,C20:5) and linoleic acid(C18:2) . The content of DHA was increased and EPA decreased significantly in middle dose group.The increase of arachidonic acid(C20:4) was not significant.Conclusion:Soybean phospholipids may improve learning and memory abilities,which might contribute to change of fatty acids contents in hippocampus of mice.
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Object To study the micro morphological characteristics of the leaf and stem epidermis of three species in Rabdosia (Bl ) Hassk Methods The leaf and stem epidermis of Rabdosia amethystoides (Benth ) Hara, R macrocalyx (Dann) Hara and R nervosa (Hemsl ) C Y Wu et H W Li were observed by scanning electron microscope (SEM) Results Some characteristics are same among three species, such as elliptical stoma and glandular scale which has four cells, but some characteristics are di fferent, such as the shape of epidermal cell, the distribution of nonglandular hair, the shape of stoma Conclusion The leaf and stem epidermal characteristics are obviously different among the three species, it provides the new reference to discriminate the plants of Rabdosia (Bl ) Hassk
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Objective: To study the effects of soybean phospholipids on fatty acids and phospholipids content in erythrocyte membranes of mice. Methods:The mice were fed with soybean phospholipids of 2 . 5, 5 . 0 , 10.0 g/kg bw respectively while control group with distilled water for 30 days. Then the mice were killed and contents of fatty acids and phospholipids in erythrocyte membranes were detected by HPLC. Results: (1)The contents of linoleic acid (C18 ∶2) and linolenic acid ( C18 ∶3), the composition of polyunsaturated fatty acid (PUFA) were increased obviously (P
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Objective: To observe the effects of soybean phospholipids on blood components in mice. Methods: Forty male mice were divided into 4 groups. Experimental mice were fed with different doses of soybean phospholipids (2.5, 5 and 10 g/kg bw) and the control mice were fed with normal saline by tube feeding for 18 days. White blood cells, red blood cells, platelets and hemoglobin as well as other blood components were measured. Results: The soybean phospholipid at dose of 10 g/kg bw could significantly increase lymphocytes, lymphocyte percentage, hemoglobin, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration, significantly decrease platelets, but did not obviously cause effects on white blood cells and red blood cells. The soybean phospholipid at dose of 5 g/kg bw could significantly increase lymphocyte percentage, hemoglobin, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration, but did not obviously affect white blood cells, red blood cells and platelets. The soybean phospholipid at dose of 2.5 g/kg bw could significantly increase mean corpuscular hemoglobin concentration, but did not obviously affect other indices of blood components. Conclusion: The soybean phospholipid can enhance immune function and increase hemoglobin and is valuable for use as a hematonic food.